METHOD FOR EXPRESSING RECOMBINANT PROTEINS USING THE EFFECT OF STRESS AND STRESS RESPONSE MECHANISMS IN THE PRESENCE OF COMPATIBLE SOLUTES

The invention relates to a method for expressing and recovering recombinant proteins using transformed organisms in cultures. The inventive method is characterized in that the transformed organisms are placed in a state of stress by changing the ambient conditions and that the production of the reco...

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Bibliographische Detailangaben
Hauptverfasser:	LOUIS, PETRA, ENGERT, ANDREAS, HUHN, MICHAEL, BARTH, STEFAN, GALINSKI, ERWIN
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for expressing and recovering recombinant proteins using transformed organisms in cultures. The inventive method is characterized in that the transformed organisms are placed in a state of stress by changing the ambient conditions and that the production of the recombinant protein in the presence of compatible solutes is triggered by an inducer. Verfahren zur Expression und Gewinnung rekombinanter Proteine durch transformierte Organismen in Kulturen, dadurch gekennzeichnet, dass die transformierten Organismen durch Änderung der Umgebungsbedingungen in einen Stresszustand versetzt werden und die Produktion des rekombinanten Proteins in Gegenwart von kompatiblen Soluten durch einen Induktor ausgelöst wird. The invention relates to a method for expressing and recovering recombinant proteins using transformed organisms in cultures. The inventive method is characterized in that the transformed organisms are placed in a state of stress by changing the ambient conditions and that the production of the recombinant protein in the presence of compatible solutes is triggered by an inducer. L'invention concerne un procédé permettant d'exprimer et d'obtenir des protéines recombinées par des organismes transformés dans des cultures. Ce procédé se caractérise en ce que les organismes transformés sont mis en état de stress par modification des conditions ambiantes. La production de la protéine recombinée est déclenchée par un inducteur, en présence de solutés compatibles.