DRUG TARGETS IN CANDIDA ALBICANS

The present invention is concerned with a method of identifying compounds which selectively modulate expression of polypeptides which are crucial for growth and survival of Candida albicans, which method comprises: (a) contacting a compound to be tested with one or more Candida albicans cells having...

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Bibliographische Detailangaben
Hauptverfasser:	VIAENE, JASMINE, ELZA, LOGGHE, MARC, GEORGE, LUYTEN, WALTER, HERMAN, MARIA, LOUIS, DE BACKER, MARIANNE, DENISE, NELISSEN, BART, CONTRERAS, ROLAND, HENRI
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is concerned with a method of identifying compounds which selectively modulate expression of polypeptides which are crucial for growth and survival of Candida albicans, which method comprises: (a) contacting a compound to be tested with one or more Candida albicans cells having a mutation in a nucleic acid molecule corresponding to the sequences according to any of claims 1 to 8 which mutation results in overexpression or underexpression of said polypeptides, in addition to contacting one or more wild type Candida albicans cells with said compound, (b) monitoring the growth and/or activity of said mutated cell compared to said wild type; wherein differential growth or activity of said one or more mutated Candida cells is indicative of selective action of said compound on a polypeptide or another polypeptide in the same or a parallel pathway. Also disclosed in the present invention are compounds identified and the sequences themselves which are critical for survival and growth of Candida albicans. L'invention porte sur un procédé d'identification de composés modulant sélectivement l'expression de polypeptides essentiels pour la croissance et la survie du Candida albicans. Ladite méthode consiste: (a) à mettre en contact le composé à tester avec une ou plusieurs cellules de Candida albicans dont une molécule d'acide nucléique présente une mutation correspondant aux séquences des revendications 1 à 8, ladite mutation se traduisant par une surexpression ou une sousexpression desdits polypeptides, et ceci après avoir mis en contact une ou plusieurs cellules de Candida albicans de type sauvage avec ledit composé; et (b) à suivre la croissance et/ou l'activité de ladite cellule mutée par rapport à celle de la cellule de type sauvage, la différence de croissance ou d'activité de la ou des cellules de Candida albicans indiquant l'action sélective dudit composé sur ce polypeptide (ou un autre) selon un mode d'action identique ou parallèle. L'invention porte également sur les composés identifiés et les séquences qui sont critiques pour la survie et la croissance du Candida albicans.