Enzymatic encoding methods for efficient synthesis of large libraries

Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reactio...

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Hauptverfasser:	Lundorf Mikkel Dybro, Holtmann Anette, Franch Thomas, Sørensen Anders Malling, Dolberg Johannes, Andersen Anne Lee, Goldbech Anne, Hansen Anders Holm, Nørregaard-Madsen Mads, Godskesen Michael Anders, Glad Sanne Schrøder, Thisted Thomas, Felding Jakob, Kronborg Tine Titilola Akinleminu, Sams Christian Klarner, Olsen Eva Kampmann, Kaldor Ditte Kievsmose, Gouliaev Alex Haahr, Husemoen Gitte Nystrup, Jacobsen Søren Nyboe, Pedersen Lene, Sløk Frank Abildgaard, Pedersen Henrik, Neve Søren, Freskgård Per-Ola, de Leon Daen, Jensen Kim Birkebæ
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).