Enhanced protein purification through a modified protein A elution

The present invention provides methods for purifying a polypeptide comprising a CH2/CH3 region, comprising binding the polypeptide to Protein A and eluting with a pH gradient starting at a low pH.

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Bibliographische Detailangaben
Hauptverfasser: Dowd Christopher J, Brown Arick, Radhamohan Asha Nandini
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention provides methods for purifying a polypeptide comprising a CH2/CH3 region, comprising binding the polypeptide to Protein A and eluting with a pH gradient starting at a low pH.