Method of detecting a biological activity

The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined bi...

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Bibliographische Detailangaben
Hauptverfasser: ENGEL BRIAN J, WEBB HEATHER M, CHANDRAPATI SAILAJA, PEDERSON JEFFREY C, HALVERSON KURT J
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.