Modified FRT recombination site libraries and methods of use

Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT r...

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Bibliographische Detailangaben
Hauptverfasser: TAO YUMIN, BIDNEY DENNIS L, GORDON-KAMM WILLIAM J, LYZNIK LESZEK A
Format: Patent
Sprache:eng
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Zusammenfassung:Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.