Compositions and methods for in vitro diagnostic tests including sulfonic acid compound

The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the ch...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: OPPERMAN GARY, NELSON WENDY
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.