Method for making mature insulin polypeptides

The invention is related to a method for making human insulin analogs by culturing an fungi cell comprising a DNA vector encoding a precursor for human insulin analog, wherein the said precursor comprises a connecting peptide flanked with cleavage sites at both junctions with the A- and the B-chain...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: CHRISTENSEN LARS HOJLUND, ANDERSEN ASSER SLOTH
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention is related to a method for making human insulin analogs by culturing an fungi cell comprising a DNA vector encoding a precursor for human insulin analog, wherein the said precursor comprises a connecting peptide flanked with cleavage sites at both junctions with the A- and the B-chain of the insulin peptide, respectively said cleavage sites being cleaved within the fungi cell allowing the cell to secrete high amount of correctly processed, mature two chain human insulin analog to the culture media.