Process for recovering polyhydroxialkanoates ("PHAs") from cellular biomass

A process for recovering polyhydroxyalkanoates (PHAs) from cellular biomass of bacteria, said biomass being obtained by fermentation and in the form of a cellular biomass slurry in aqueous suspension, comprising the steps of: submitting the slurry to operations of injection of PHA solvent, agitation...

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Bibliographische Detailangaben
Hauptverfasser:	ROCCHICCIOLI CARLO, KESSERLINGH SONIA MARIA, SATO TETUHIKO, NONATO ROBERTO VIANNA, MANTELATTO PAULO EDUARDO, DUZZI ALVARO MINTO, DURAO NAZARENO ANTONIO SERTORI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process for recovering polyhydroxyalkanoates (PHAs) from cellular biomass of bacteria, said biomass being obtained by fermentation and in the form of a cellular biomass slurry in aqueous suspension, comprising the steps of: submitting the slurry to operations of injection of PHA solvent, agitation and heating, in order to form a suspension comprising PHA solvent with the dissolved PHA, water and insoluble residues; recuperating the solvent enriched with PHA; rapidly cooling the solution of PHA solvent to precipitate the dissolved PHA; micro-filtrating the suspension of PHA precipitated in the solvent, in order to separate a paste concentrated with precipitated PHA; washing with water, heating and agitating the concentrated PHA paste, to promote evaporation of the solvent and to obtain a suspension containing PHA granules; agitating and shearing the PHA granules and depleting the residual solvent; and separating the purified PHA particles from the suspension.