Nitrilases and methods for making and using them

The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure alpha-substituted carboxylic acids, such as, for example, alpha-amino acids and alpha-hydroxy acids. In one aspect, methods of the invention combine an ald...

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Bibliographische Detailangaben
Hauptverfasser:	WEINER DAVID PAUL, ROBERTSON DAN E, MADDEN MARK, CHAPLIN JENNIFER ANN
Format:	Patent
Sprache:	eng
Schlagworte:	ACYCLIC OR CARBOCYCLIC COMPOUNDS BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure alpha-substituted carboxylic acids, such as, for example, alpha-amino acids and alpha-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.