Proteases and methods for producing them

A secreted mature polypeptide derived from an S2A or S1E protease is described which after maturation has protease activity, which polypeptide when expressed and before maturation comprises a heterologous pro-region. Further, a method for producing polypeptide is described including the step of cult...

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1. Verfasser:	LASSEN SOREN FLENSTED
Format:	Patent
Sprache:	eng
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A secreted mature polypeptide derived from an S2A or S1E protease is described which after maturation has protease activity, which polypeptide when expressed and before maturation comprises a heterologous pro-region. Further, a method for producing polypeptide is described including the step of cultivating a recombinant host cell including a polynucleotide encoding a secreted mature polypeptide which after maturation has protease activity, which polypeptide when expressed and before maturation includes a heterologous pro-region derived from an S2A or S1E protease to produce a supermatant including the polypeptide. The polypeptide may also be recovered.