System and method for performing microfluidic manipulation

Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.