Recombinant Candida rugosa lipases

The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons correspondin...

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Bibliographische Detailangaben
Hauptverfasser:	SHAW JEI-FU, LEE GUANIUN, TANG SHYE-JYE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention features an isolated nucleic acid that includes a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 (e.g., 13, 15, 17, or all) universal serine codons corresponding to CTG codons in the wild-type DNA. Each of the universal serine codons, independently, is TCT, TCC, TCA, TCG, AGT, or AGC. The Candida rugosa lipase can be Candida rugosa lipase 1, 2, 3, 4, 5, or 8.