Method for converting sucrose to beta-D-glucose

Method for converting sucrose to beta-D-glucose

Disclosed is an apparatus and method for continuously converting sucrose to beta-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and alpha-D-glucose by passing it through a porous, packed column co...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: VOLPONI JOANNE V, WALKER ANDREW, SIMMONS BLAKE A, INGERSOLL DAVID
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Disclosed is an apparatus and method for continuously converting sucrose to beta-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and alpha-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.