Serum-free medium for producing retroviruses

A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a st...

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Hauptverfasser: FUNAKOSHI MASAO, TOMURA DAISUKE, MATSUMOTO KAZUYA, KATAYAMA YASUSHI, MINENO JUNICHI, KATO IKUNOSHIN, YOSHIOKA HIROFUMI, TAKESAKO KAZUTOH
Format: Patent
Sprache:eng
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Zusammenfassung:A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.