Method for detection of base sequence of interest

There is provided a method for the detection of a base sequence of interest when amount of a sample DNA or RNA is little and plural base sequences of interest to be detected are present in the sample DNA or RNA. The Problem is solved by a method for the detection of an base sequence of interest in a...

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Bibliographische Detailangaben
Hauptverfasser: MUGITA AKITO, KOBAYASHI TAKANORI, ONO HIROAKI, NAKAYAMA ICHIRO, NASU HISANORI, MASAOKA TETSUJI, KUHARA SATORU, ISHINO YOSHIZUMI
Format: Patent
Sprache:eng
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Zusammenfassung:There is provided a method for the detection of a base sequence of interest when amount of a sample DNA or RNA is little and plural base sequences of interest to be detected are present in the sample DNA or RNA. The Problem is solved by a method for the detection of an base sequence of interest in a sample DNA or RNA comprising the steps of (1) contacting a sample DNA or RNA to a probe DNAs or RNAs in an aqueous solution to form a hybridization complex; (2) isolating the hybridization complex; (3) dissociating the complex to recover the probe DNAs or RNAs; and (4) identifying the said probe DNAs or RNAs to detect an base sequence of interest in the sample DNA or RNA.