Process for reducing an alpha-keto ester

A process for preparing (R)-hydroxy ester (II) from alpha-keto ester I comprising adding the alpha-keto ester I to a mixture comprising the ketoreductase enzyme and non-ketoreductase enzyme components, wherein the ketoreductase enzyme has a molecular weight between 36000 and 38000, and wherein the k...

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Bibliographische Detailangaben
Hauptverfasser:	STURR MICHAEL G, KIM JAEHON, MOORE JEFFREY C, MCLAUGHLIN KATHLEEN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process for preparing (R)-hydroxy ester (II) from alpha-keto ester I comprising adding the alpha-keto ester I to a mixture comprising the ketoreductase enzyme and non-ketoreductase enzyme components, wherein the ketoreductase enzyme has a molecular weight between 36000 and 38000, and wherein the ketoreductase enzyme has an N-terminal amino acid sequence selected from the group of sequences consisting of (SEQ. ID NO. 1) Ala-Ile-Pro-Asp-Asn-Ala-Val-Leu-Glu-Gly-Ser-Leu- Val-Lys-Val-Thr-Gly-Ala-Asn-Gly (SEQ. ID NO. 4) Met-Ala-Ile-Pro-Asp-Asn-Ala-Val-Leu-Glu-Gly-Ser- Leu-Val-Lys-Val-Thr-Gly-Ala-Asn-Gly, and (SEQ. ID NO. 2) Met-Ala-Lys-Ile-Asp-Asn-Ala-Val-Leu-Pro-Glu-Gly- Ser-Leu-Val-Leu-Val-Thr-Gly-Ala-Asn-Gly.