Test piece and quantitative method and apparatus for an organism-oriented substance

A plurality of cDNAs each having a known different base sequence are labeled with a fluorescent dye (FITC), whereby F-cDNA is prepared. The F-cDNA is disposed at a plurality of predetermined positions on the slide glass of a DNA micro array chip. Cy5-cDNA is prepared by synthesizing cDNA from poly(A...

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Bibliographische Detailangaben
1. Verfasser:	AKIMOTO TAIZO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A plurality of cDNAs each having a known different base sequence are labeled with a fluorescent dye (FITC), whereby F-cDNA is prepared. The F-cDNA is disposed at a plurality of predetermined positions on the slide glass of a DNA micro array chip. Cy5-cDNA is prepared by synthesizing cDNA from poly(A)-mRNA in the presence of a fluorescent dye (Cy5). The Cy5-cDNA is placed on the DNA micro array chip and hybridized to the F-cDNA. After hybridization, the DNA micro array chip is read by a quantitative apparatus incorporated with an analyzer in which information about the base composition ratio and base length of cDNA has been registered.