Nucleic acid amplification

Disclosed is an insoluble support that can be used, for example, for producing replicates of sample nucleic acids. The support includes a plurality of attached oligonucleotides that include a prokaryotic promoter sequence and a target annealing sequence 3' of the promote. The proximal end of th...

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Bibliographische Detailangaben
Hauptverfasser: LIAO HAISUN, MAMAEVA NATALIA, CHEN SHIN-YIH, DEIK AMY ANDERSON, LAW SIMON W, WOODWARD CAROLINE NGAARA, HUANG YIH, HUANG TAI-NANG, SHEN MING
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Disclosed is an insoluble support that can be used, for example, for producing replicates of sample nucleic acids. The support includes a plurality of attached oligonucleotides that include a prokaryotic promoter sequence and a target annealing sequence 3' of the promote. The proximal end of the promoter sequence is spaced from the insoluble support by a distance greater than 10 nm.