Biological activity probe

A biological activity probe for the detection on-line of the presence of sulphide including an enzyme electrode; a counter electrode; a reference electrode; and if desired a corrosion electrode. The enzyme electrode includes an immobilised source of sulfide oxidase enzyme, together with a cofactor s...

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Bibliographische Detailangaben
Hauptverfasser:	PAPAVINASAM SADAKSHARASUNDARAM S, REVIE ROBERT W, ATTARD MICHAEL, GOULD WILLIAM D, MACLEOD FRASER A
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A biological activity probe for the detection on-line of the presence of sulphide including an enzyme electrode; a counter electrode; a reference electrode; and if desired a corrosion electrode. The enzyme electrode includes an immobilised source of sulfide oxidase enzyme, together with a cofactor such as 1,1'-dimethylferrocene, immobilised in a water permeable non-conducting binder on a biologically inert electrically conducting substrate. The immobilised source of sulfide oxidase enzyme is either a chemoautotropic microorganism such as a Thiobacillus thioparus, or enzyme material recovered from a culture of a Thiobacillus thioparus. The probe when connected to a suitable electrical system is capable of identifying microbiological activity resulting in microbiologically influenced corrosion; microbiological activity not resulting in microbiologically influenced corrosion; microbiological inactivity, coupled with non-microbiologically influenced corrosion; and microbiological inactivity; and no corrosion.