Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis

The present invention relates to subtilisin Carlsberg variants having a modified amino acid sequence of wild-type subtilisin Carlsberg amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop...

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Bibliographische Detailangaben
Hauptverfasser:	BARNETT BOBBY LEE, BRODE, III PHILIP FREDERICK, RUBINGH DONN NELTON
Format:	Patent
Sprache:	eng
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to subtilisin Carlsberg variants having a modified amino acid sequence of wild-type subtilisin Carlsberg amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin Carlsberg (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the subtilisin Carlsberg variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin Carlsberg. The present invention also relates to the genes encoding such subtilisin Carlsberg variants. The present invention also relates to compositions comprising such subtilisin Carlsberg variants for cleaning a variety of surfaces.