Pullulanase expression constructs containing alpha-amylase promoter and leader sequences

Pullulanase expression constructs containing alpha-amylase promoter and leader sequences

Disclosed herein are DNA expression constructs containing an alpha-amylase promoter sequence derived from Bacillus stearothermophilus, an alpha-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hos...

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Hauptverfasser: ALLEN LARRY N, TEAGUE W. MARTIN, BRUMM PHILLIP J, BRIKUN IGOR A
Format: Patent
Sprache:eng
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator ALLEN LARRY N
TEAGUE W. MARTIN
BRUMM PHILLIP J
BRIKUN IGOR A
description Disclosed herein are DNA expression constructs containing an alpha-amylase promoter sequence derived from Bacillus stearothermophilus, an alpha-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hosts transformed to contain the expression constructs secret function pullulanases. Also disclosed is a process for making recombinant pullulanases utilizing the expression constructs and a recombinant pullulanase which can be produced in Bacillus subtilis.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Pullulanase expression constructs containing alpha-amylase promoter and leader sequences
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