Pullulanase expression constructs containing alpha-amylase promoter and leader sequences

Pullulanase expression constructs containing alpha-amylase promoter and leader sequences

Disclosed herein are DNA expression constructs containing an alpha-amylase promoter sequence derived from Bacillus stearothermophilus, an alpha-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hos...

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Bibliographische Detailangaben
Hauptverfasser: ALLEN LARRY N, TEAGUE W. MARTIN, BRUMM PHILLIP J, BRIKUN IGOR A
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Disclosed herein are DNA expression constructs containing an alpha-amylase promoter sequence derived from Bacillus stearothermophilus, an alpha-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hosts transformed to contain the expression constructs secret function pullulanases. Also disclosed is a process for making recombinant pullulanases utilizing the expression constructs and a recombinant pullulanase which can be produced in Bacillus subtilis.