Method for detecting nucleic acid methylation using AFLP(TM)

The invention relates to a method for analyzing of determining the methylation pattern of a starting DNA and/or for distinguishing between methylated and non-methylated sites in the starting DNA, comprising at least(A) generating a first DNA fingerprint, containing bands corresponding to both the me...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	VOS PETRUS ANTONIUS JOSEPHINA, ZABEAU MARCUS FLORENT OSCAR, VUYLSTEKE MARNIK JOHAN ROGER
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for analyzing of determining the methylation pattern of a starting DNA and/or for distinguishing between methylated and non-methylated sites in the starting DNA, comprising at least(A) generating a first DNA fingerprint, containing bands corresponding to both the methylated and non-methylated sites of interest; and/or(B) generating a second DNA fingerprint, containing bands corresponding only to the methylated sites of interest; and optionally comprising(C) generating a third DNA fingerprint, containing bands corresponding only to the non-methylated sites of interest; and optionally further comprising(D) analysing the fingerprint(s) thus obtained. The fingerprints are preferably generated using AFLP, by means of a frequent cutter and a methylation sensitive rare cutter. The invention further relates to specific methods for generating the above first and second DNA fingerprint by means of AFLP, and kits for use with said methods.