Internally controlled virion nucleic acid amplification reaction for quantitation of virion and virion nucleic acid

The invention relates generally to the quantitation of virus and viral nucleic acid. The invention relates to methods for quantitating an amount of virus present in a sample, comprising introducing into the sample a composition comprising a genetically tagged viral nucleic acid, isolating said wild...

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Bibliographische Detailangaben
Hauptverfasser: SALZMAN, NORMAN P, NATARAJAN, VENKATACHALA
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention relates generally to the quantitation of virus and viral nucleic acid. The invention relates to methods for quantitating an amount of virus present in a sample, comprising introducing into the sample a composition comprising a genetically tagged viral nucleic acid, isolating said wild type and said tagged nucleic acid, and quantitating said wild type and said tagged nucleic acid. The invention also relates to genetically tagged retroviral nucleic acid comprising a tag sequence, including insertions and deletions.