Relating to assay systems

PCT No. PCT/GB96/03172 Sec. 371 Date Aug. 25, 1998 Sec. 102(e) Date Aug. 25, 1998 PCT Filed Dec. 20, 1996 PCT Pub. No. WO97/23609 PCT Pub. Date Jul. 3, 1997A method for detecting an interaction between two proteins while eliminating false positive interactions, the method being particularly useful f...

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Hauptverfasser: JONES, NICHOLAS, HURD, DOUGLAS, FALLON, RACHEL ALISON, WHITE, MICHAEL
Format: Patent
Sprache:eng
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Zusammenfassung:PCT No. PCT/GB96/03172 Sec. 371 Date Aug. 25, 1998 Sec. 102(e) Date Aug. 25, 1998 PCT Filed Dec. 20, 1996 PCT Pub. No. WO97/23609 PCT Pub. Date Jul. 3, 1997A method for detecting an interaction between two proteins while eliminating false positive interactions, the method being particularly useful for application in the two-hybrid system and for the screening of libraries of unknown proteins. In a two-hybrid system employing the method, two different repoter genes having different promoters and two different DNA-binding domains which specifically bind to those promoters, are used.