Luminescence method for luciferin/luciferase system and luminescent reagent

PCT No. PCT/JP98/03833 Sec. 371 Date Apr. 30, 1999 Sec. 102(e) Date Apr. 30, 1999 PCT Filed Aug. 28, 1998 PCT Pub. No. WO99/11766 PCT Pub. Date Mar. 11, 1999A luminescence method for a luciferin/luciferase system which comprises reacting a luciferin solution containing an increased amount of dissolv...

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Hauptverfasser: TAKEUCHI, HIDEYUKI, RYUFUKU, MASAYUKI, TANAKA, HOZUMI
Format: Patent
Sprache:eng
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Zusammenfassung:PCT No. PCT/JP98/03833 Sec. 371 Date Apr. 30, 1999 Sec. 102(e) Date Apr. 30, 1999 PCT Filed Aug. 28, 1998 PCT Pub. No. WO99/11766 PCT Pub. Date Mar. 11, 1999A luminescence method for a luciferin/luciferase system which comprises reacting a luciferin solution containing an increased amount of dissolved carbon dioxide with luciferase; and a luciferase assay reagent comprising a luciferin solution containing an increased amount of dissolved carbon dioxide. Thus, a method of luminescent assay excellent in continuity and stability is provided as a substitute for the conventional luminescent luciferase assay featured by rapidity and high sensitivity.