Octanucleotide restriction endonuclease, Srf I, and method for producing same

This invention provides restriction endonuclease Srf I, which is capable of recognizing the eight nucleotide base palindromic sequence shown below on a double stranded DNA molecule and cleaving the DNA chain at the asterisk-marked position resulting in blunt ends - 5'GCCC*GGGC3' - 3'C...

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Bibliographische Detailangaben
Hauptverfasser:	SIMCOX, TIMOTHY G, MARY E
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention provides restriction endonuclease Srf I, which is capable of recognizing the eight nucleotide base palindromic sequence shown below on a double stranded DNA molecule and cleaving the DNA chain at the asterisk-marked position resulting in blunt ends - 5'GCCCGGGC3' - 3'CGGGCCCG5' - (wherein G and C respectively represent the nucleotides guanine and cytosine). The restriction endonuclease is produced by culturing Streptomyces rf in a culture medium and recovering it from the culture. The invention also provides polynucleotides encoding Srf I. Another aspect of this invention is to provide for the recombinant DNA production of Srf I. Another aspect of the invention is to provide for non-naturally occurring variants of Srf I, as well as the polynucleotides encoding these proteins.