Hepatitis C virus second envelope (HCV-E2) glycoprotein expression system

This invention provides a novel mammalian expression system that is capable of generating high levels of expressed hepatitis C virus (HCV) proteins which have previously proved difficult to express due to their non-secretory properties. In particular, the invention provides a plasmid for the express...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	OKASINSKI, GREGORY F, LESNIEWSKI, RICHARD R, SCHAEFER, VERLYN G, SUHAR, THOMAS S
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMISTRY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS TESTING
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Beschreibung
Zusammenfassung:	This invention provides a novel mammalian expression system that is capable of generating high levels of expressed hepatitis C virus (HCV) proteins which have previously proved difficult to express due to their non-secretory properties. In particular, the invention provides a plasmid for the expression of the HCV second envelope protein (E2) designated p577. This plasmid encodes a recombinant protein comprising the immunoglobulin signal peptide and amino acids 388-664 of the HCV E2 glycoprotein. This unique expression system produces high levels of HCV proteins that are properly processed, glycosylated, and folded.