Method and device for rendering cells observable with laser scanning cytometry

A laser scanning cytometer, used with forward light scatter measurements, is provided with a laterally extended, asymmetrically positioned (relative to a light blocking position) light blocker, positioned between a cell sample and a detector such as a photodiode. The light blocker is asymmetrically...

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Hauptverfasser: LUTHER, EDGAR A
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A laser scanning cytometer, used with forward light scatter measurements, is provided with a laterally extended, asymmetrically positioned (relative to a light blocking position) light blocker, positioned between a cell sample and a detector such as a photodiode. The light blocker is asymmetrically positioned, at the reference position (i.e., no specimen) to permit some light to pass by a first end thereof and wherein light scatter caused by a specimen cell passes the first end, with other light scatter being blocked by the extended light blocker at a second end. Contrast of light scatter detection is sufficient thereby to provide viewable images of cells by means of the laser scanning cytometer.