Processes for preparing acarviosyl transferase and for using it in the conversion of acarbose homologues into acarbose, for the preparation of acarbose homologues

The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expressi...

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Hauptverfasser:	PIEPERSBERG, WOLFGANG, SCHROEDER, WERNER, CRUEGER, ANNELIESE, DELLWEG, HANS-GEORG, SCHAPER, BEATE, STRATMANN, ANSGAR, LENZ, JUERGEN GEORG, GOEKE, KLAUS, HEMKER, MICHAEL, DISTLER, JUERGEN, PAPE, HERMANN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.