Methods of assessing viability of microbial cultures

The present invention provides methods for rapidly determining the proportion of live, dead and stressed cells in a given microbial culture. The measurement of stressed cells is used as a quantitative indicator of the relative health of the population and the population's ability to withstand l...

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Bibliographische Detailangaben
Hauptverfasser:	RUTHERFORD, WILLIAM MICHAEL, HARMAN, ELIZABETH K
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides methods for rapidly determining the proportion of live, dead and stressed cells in a given microbial culture. The measurement of stressed cells is used as a quantitative indicator of the relative health of the population and the population's ability to withstand long term stress. The present methods comprise the steps of (i) staining the culture with a combination of membrane-permeable and membrane-impermeable stains; and (ii) quantifying the population of stressed cells, using the measurement thus obtained as an indicator of the relative health of the population.