RAS antisense inhibition

A single stranded oligonucleotide complementary to a specific region of a gene promoter, methylated at carbon 5 of all cytosine residues in cytosine-guanine (CG) dinucleotides, is provided. The modified oligonucleotide provides DNA methyltransferase (MTase) enzyme with a hemimethylated substrate so...

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Bibliographische Detailangaben
Hauptverfasser:	SHEIKHNEJAD, GHOLAMREZA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A single stranded oligonucleotide complementary to a specific region of a gene promoter, methylated at carbon 5 of all cytosine residues in cytosine-guanine (CG) dinucleotides, is provided. The modified oligonucleotide provides DNA methyltransferase (MTase) enzyme with a hemimethylated substrate so that DNA MTase can methylate complementary CG sites using methylated oligonucleotides as the template. Such methylation prevents translation of the gene. An oligonucleotide complementary to the sense strand of c-Ha-ras promoter having a methyl group on all cytosine residues of its 6 CpG sites was specifically effective in inhibiting breast tumor cell proliferation and tumorigenicity.