Stable expression of human A2B adenosine receptors, and assays employing the same

Stable expression of human A2B adenosine receptors, and assays employing the same

Stable over-expression of native and hexahistidine/FLAG extended recombinant human adenosine receptors permits recovery of membranes and membrane fragments bearing a high density of adenosine receptors. Cell lines expressing all four sub-types of human adenosine receptors have been identified. The r...

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Bibliographische Detailangaben
Hauptverfasser: JIN, XIAOWEI, LINDEN, JOEL, TAYLOR, HEIDI, WOODARD, ROBIN, ROBEVA, ANNA
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Zusammenfassung:Stable over-expression of native and hexahistidine/FLAG extended recombinant human adenosine receptors permits recovery of membranes and membrane fragments bearing a high density of adenosine receptors. Cell lines expressing all four sub-types of human adenosine receptors have been identified. The receptors, and membranes and membrane fragments bearing the same, can be used in assays to screen compounds for binding ability to the adenosine receptors, such as potential pharmaceutical agents.