Apparatus and method for shear breakage of polynucleotides

Apparatus and method for producing a defined size distribution of polynucleotide fragments having a selected mean size range between about 100 and 20,000 base pairs. The method includes passing a suspension of polynucleotide fragments through an apparatus containing a capillary with an orifice near...

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Hauptverfasser: HUNICKE-SMITH, SCOTT P, OEFNER, PETER JOSEF
Format: Patent
Sprache:eng
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Zusammenfassung:Apparatus and method for producing a defined size distribution of polynucleotide fragments having a selected mean size range between about 100 and 20,000 base pairs. The method includes passing a suspension of polynucleotide fragments through an apparatus containing a capillary with an orifice near the outlet end of the capillary.