Purification of recombinant human IL-1 alpha

A process for the production of essentially homogeneous soluble, stable, endotoxin free human recombinant interleukin-1 alpha of enhanced specific activity is described. The process involves breaking transformed microbial cells containing expressed human interleukin-1 alpha and separating the solubl...

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Hauptverfasser:	STERN, ALVIN SETH
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process for the production of essentially homogeneous soluble, stable, endotoxin free human recombinant interleukin-1 alpha of enhanced specific activity is described. The process involves breaking transformed microbial cells containing expressed human interleukin-1 alpha and separating the soluble supernatant from the insoluble cell components and then passing the supernatant through gel chromatography and ion-exchange chromatography steps.