N-terminal protein sequencing reagents and methods which form amino acid detectable by a variety of techniques

N-terminal protein sequencing reagents and methods which form amino acid detectable by a variety of techniques

PCT No. PCT/US94/07785 Sec. 371 Date Apr. 11, 1997 Sec. 102(e) Date Apr. 11, 1997 PCT Filed Jul. 8, 1994 PCT Pub. No. WO96/02003 PCT Pub. Date Jan. 25, 1996Novel alkoxythiocarbonylimidazoles provide new reagents for the N-terminal sequencing of small polypeptide samples. These reagents form an alkox...

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Hauptverfasser: BAILEY, JEROME
Format: Patent
Sprache:eng
Schlagworte:
ACYCLIC OR CARBOCYCLIC COMPOUNDS
CHEMISTRY
HETEROCYCLIC COMPOUNDS
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Zusammenfassung:PCT No. PCT/US94/07785 Sec. 371 Date Apr. 11, 1997 Sec. 102(e) Date Apr. 11, 1997 PCT Filed Jul. 8, 1994 PCT Pub. No. WO96/02003 PCT Pub. Date Jan. 25, 1996Novel alkoxythiocarbonylimidazoles provide new reagents for the N-terminal sequencing of small polypeptide samples. These reagents form an alkoxy thiourea derivative which is cleaved with acid to remove the N-terminal amino acid as a stable thiazolinone which does not rearrange to a thiohydantoin. This thiazolinone may be derivatized to provide a detectable group such as a fluorescent group or ionizable group detectable by mass spectrometry.