Deprotection of RNA with alkylamine

A method for the deprotection of RNA comprising reacting an alkylamine or ammonium hydroxide/alkylamine mixture with the RNA at 60-70 degrees Centigrade for 5 to 15 minutes in order to remove any exocyclic protecting groups, wherein said alkylamine is selected from the group consisting of ethylamine...

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Bibliographische Detailangaben
Hauptverfasser:	USMAN, NASSIM, TRACZ, DANUTA, WINCOTT, FRANCINE E
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for the deprotection of RNA comprising reacting an alkylamine or ammonium hydroxide/alkylamine mixture with the RNA at 60-70 degrees Centigrade for 5 to 15 minutes in order to remove any exocyclic protecting groups, wherein said alkylamine is selected from the group consisting of ethylamine, propylamine, and butylamine.