Digital subtraction angiography for 3D diagnostic imaging

The digital subtraction angiography method useful for three dimensional (3D) imaging of a selected volume of a body comprises the following steps. Acquiring first and second 3D data sets representative of an image of substantially the same selected volume in the body, the first and second data sets being acquired at different times corresponding to a pre- and a post injection of a contrast medium, respectively. Determining common reference points for spatially corresponding subvolumes in the data sets. Comparing in a 3D spatial manner data in subvolumes of the second data set with data in corresponding subvolumes in the first data set in order to determine a new reference point in each of the subvolumes of the first data set which results in a best match of the spatial similarity of the data in the corresponding subvolumes of the second data set. Spatially interpolating new data for the subvolumes of said first data set using the new reference points determined above and the originally acquired data of the first data set, thereby generating a new first data set for the volume. Subtracting the new first data set from said second data set, for generating a subtraction data set representative of a 3D image of said selected volume in which said given portion is accentuated.