Method and apparatus for analytical determination of glucose in a biological matrix

A method for determining glucose concentration in a biological matrix includes the steps of performing at least two detection measurements. In each detection measurement light is irradiated at a defined irradiation site as primary light into the biological matrix, through a boundary surface thereof. The light is propagated along a light path within the biological matrix. An intensity of the light is measured as the light emerges as secondary light through a defined detection site of the boundary surface. At least one of the detection measurements is a spatially resolved measurement of multiply scattered light. The detection site is located relative to the irradiation site such that light which was multiply scattered at scattering centers in the biological matrix is detected. The light paths of the at least two detection measurements within the biological matrix are different. Glucose concentration is then derived from the dependence of the intensity of the secondary light from the relative position of the irradiation site and the detection site.