Expression cloning of c-src SH3-domain binding proteins

Expression cloning of c-src SH3-domain binding proteins

This invention provides a unique SH3 binding domain core motif of the sequence RPLPXXP and cDNA clones encoding proteins which interact with the SH3 domain of c-src, as well as the amino acid sequences which mediate this binding. Another embodiment of this invention is a method of identifying SH3-bi...

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Hauptverfasser: CHEADLE, CHRISTOPHER, FRENCH, STEPHEN M, RICCA, GEORGE A, JAYE, MICHAEL C, SOUTH, VICTORIA J, IVASHCHENKO, YURI D, SEARFOSS, III, GEORGE H
Format: Patent
Sprache:eng
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CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Zusammenfassung:This invention provides a unique SH3 binding domain core motif of the sequence RPLPXXP and cDNA clones encoding proteins which interact with the SH3 domain of c-src, as well as the amino acid sequences which mediate this binding. Another embodiment of this invention is a method of identifying SH3-binding proteins and elucidating the sequences which mediate binding. This method may be used as an assay to select compounds which bind to this site and which inhibit or enhance the binding of the SH3 domain.