Apparatus and process for continuous in vitro synthesis of proteins

An apparatus and process for continuous, cell-free, in vitro synthesis of peptides, particularly peptide-MHC complexes make use of a novel bioreactor flow cell which allows for the reproducible, systematic variation of single parameters in order to optimize translation processes. The bioreactor flow...

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Bibliographische Detailangaben
Hauptverfasser:	MOZAYENI, BOBAK R
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	An apparatus and process for continuous, cell-free, in vitro synthesis of peptides, particularly peptide-MHC complexes make use of a novel bioreactor flow cell which allows for the reproducible, systematic variation of single parameters in order to optimize translation processes. The bioreactor flow cell includes a pair of substantially parallel membranes positioned within a chamber to define therebetween a space, the ratio of total membrane surface area to chamber volume being at least 5 units-1, which permits high perfusion rates through the system with lower flux rate per membrane area. The apparatus also may include a novel countercurrent flow dialysis cell and other components.