Cloned N-carbamoyl sarcosine amidohydrolase

The invention concerns a recombinant DNA which contains (1) the sequence shown in SEQ ID NO: 1, (2) a sequence corresponding to this sequence within the scope of the degeneracy of the genetic code or (3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent hybridizing cond...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	SCHUMACHER, GUENTHER, BURTSCHER, HELMUT
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention concerns a recombinant DNA which contains (1) the sequence shown in SEQ ID NO: 1, (2) a sequence corresponding to this sequence within the scope of the degeneracy of the genetic code or (3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent hybridizing conditions whereby the DNA sequence codes for a protein with N-carbamoyl sarcosine amidohydrolase activity. Furthermore the invention concerns a recombinant vector with the DNA according to the present invention as well as a process for the isolation of a recombinant protein with N-carbamoyl sarcosine amidohydrolase activity and its use for the determination of creatinine.