Mutant microbial alpha -amylases with increased thermal, acid and/or alkaline stability

PCT No. PCT/EP90/01042 Sec. 371 Date Dec. 2, 1990 Sec. 102(e) Date Dec. 2, 1990 PCT Filed Jun. 27, 1990 PCT Pub. No. WO91/00353 PCT Pub. Date Jan. 10, 1991.Thermostable and acid stable alpha -amylases are provided as expression products of genetically engineered alpha -amylase genes isolated from mi...

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Hauptverfasser: VOLLEBREGT, ADRIANUS W. H, QUAX, WILHELMUS J, LAROCHE, YVES, STANSSENS, PATRICK, LAUWEREYS, MARC
Format: Patent
Sprache:eng
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Zusammenfassung:PCT No. PCT/EP90/01042 Sec. 371 Date Dec. 2, 1990 Sec. 102(e) Date Dec. 2, 1990 PCT Filed Jun. 27, 1990 PCT Pub. No. WO91/00353 PCT Pub. Date Jan. 10, 1991.Thermostable and acid stable alpha -amylases are provided as expression products of genetically engineered alpha -amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant alpha -amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.