Fluorogenic and chromogenic beta -lactamase substrates

Chromogenic and fluorogenic substrates for beta -lactamase, methods for synthesis thereof and methods for detecting beta -lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent beta -lactam compounds which include an electronegative leaving gro...

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Bibliographische Detailangaben
Hauptverfasser:	MILLNER, DECEASED, O. ELMO, QUANTE, J. MICHAEL, WOODARD, DANIEL L, MIZE, PATRICK D, HOKE, RANDAL A
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Chromogenic and fluorogenic substrates for beta -lactamase, methods for synthesis thereof and methods for detecting beta -lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent beta -lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by beta -lactamase, the leaving group is liberated and fluorescence or a colored product is produced.