Analytical method for the selective determination of sialic glycolipid complexes

PCT No. PCT/HU89/00021 Sec. 371 Date Jun. 3, 1991 Sec. 102(e) Date Jun. 3, 1991 PCT Filed May 12, 1989 PCT Pub. No. WO90/08317 PCT Pub. Date Jul. 26, 1990.A method for the selective determination of sialic acid-carrying glycolipid complexes in blood, in which the serum is diluted with water, then ex...

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Hauptverfasser:	GESZTESI NEE KOSZEGI, ANNA, KINCZEL, EDIT, SIKLOSI NEE PATAY, IVONY NEE KLADIVA, KATALIN, SZAJANI, BELA, MILOVAN, IREN, BABARCZI, JOLAN, PRIBEK, FERENC, SCHUMANN
Format:	Patent
Sprache:	eng
Schlagworte:	GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS TESTING
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Zusammenfassung:	PCT No. PCT/HU89/00021 Sec. 371 Date Jun. 3, 1991 Sec. 102(e) Date Jun. 3, 1991 PCT Filed May 12, 1989 PCT Pub. No. WO90/08317 PCT Pub. Date Jul. 26, 1990.A method for the selective determination of sialic acid-carrying glycolipid complexes in blood, in which the serum is diluted with water, then extracted with a water-immiscible organic solvent or solvent mixture to remove neutral lipids, a lipoprotein fraction containing sialic acid-carrying glycolipids is precipitated from the aqueous phase, the precipitated fraction is redissolved, and the sialic acid content of the solution or the amount and quality of the sialic acid-carrying glycolipid are determined by a method known per se. A water-soluble salt of a monovalent, bivalent or trivalent metal other than alkali metal is used as a precipitating agent in an amount corresponding to a final metal ion concentration of 0.005-0.1 mole/l in the mixture, and precipitation is performed at a pH of 1.5 to 8.0.