Method to produce recombinant proteins using an expression vector comprising transcriptional and translational activating sequences

The present invention is composed of novel recombinant DNA expression vectors which contain a transcriptional activating sequence, a translational activating sequence and a DNA sequence coding for a functional polypeptide, especially bovine growth hormone. The aforementioned translational activating...

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Bibliographische Detailangaben
Hauptverfasser:	SCHONER, RONALD G, BRIGITTE E
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is composed of novel recombinant DNA expression vectors which contain a transcriptional activating sequence, a translational activating sequence and a DNA sequence coding for a functional polypeptide, especially bovine growth hormone. The aforementioned translational activating sequences contain a ribosome binding site and are designed to provide high level expression of DNA that codes for virtually any functional polypeptide. The invention further provides transformed microbial host cells capable of producing bovine growth hormone and other functional polypeptides at high levels.