Automatic air temperature cycler and method of use in polymerose chain reaction

Apparatus and method for automatically developing, maintaining and repetitively duplicating a selectable predetermined temperature profile for replicating and amplifying a sequence of a stretch of DNA or RNA through use of a polymerase. An array of sample-containing vessels is supported in a reactio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	HALLSBY, G. ANDERS
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY ENZYMOLOGY METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SPIRITS TRANSPORTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Apparatus and method for automatically developing, maintaining and repetitively duplicating a selectable predetermined temperature profile for replicating and amplifying a sequence of a stretch of DNA or RNA through use of a polymerase. An array of sample-containing vessels is supported in a reaction chamber through which a heat transfer medium in heat-exchange relationship with the vessels. The temperature of the air is controlled as a function of time to provide a preselectable sequence defining a temperature profile. The profile is cyclically repetitively reproduced to effect amplification of the desired sequence of DNA or RNA.