Bacteriophage cloning system for the construction of directional DNA libraries

Bacteriophage cloning system for the construction of directional DNA libraries

A bacteriophage directional cloning vector containing a multiple cloning site, a method for preparing cDNAs that can be cloned in a unique orientation and a bifunctional oligodeoxynucleotide linker, which creates a unique restriction site when ligated to the 3' end of cDNA fragments. The simple...

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Bibliographische Detailangaben
Hauptverfasser: SISK, WILLIAM P, MEISSNER, P. SCOTT, BERMAN, MICHAEL L
Format: Patent
Sprache:eng
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BIOCHEMISTRY
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Beschreibung
Zusammenfassung:A bacteriophage directional cloning vector containing a multiple cloning site, a method for preparing cDNAs that can be cloned in a unique orientation and a bifunctional oligodeoxynucleotide linker, which creates a unique restriction site when ligated to the 3' end of cDNA fragments. The simple and efficient cDNA cloning system reduces the amount of RNA and effort required for the preparation of large directionally cloned libraries by providing a unique vector that accepts cDNA fragments in a unique 5'-3' orientation and by providing a unique bifunctional linker that facilitates the preparation of cDNA fragments that can be ligated directionally into the vector.