Process and reagent for the determination of alpha -amylase

A process and reagent are described for the determination of alpha -amylase by enzymatic splitting of an alpha -amylase substrate in the presence of alpha -glucosidase and beta -glucosidase and measurement of a fission product. The substrate utilized is a 4-nitrophenyl- beta ,D-maltoheptaoside subst...

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Hauptverfasser:	DOLABDJIAN, BARKEW, HELGER, ROLAND, HENKEL, EBERHARD, KLINK, RAINER, WUERZBURG, UWE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process and reagent are described for the determination of alpha -amylase by enzymatic splitting of an alpha -amylase substrate in the presence of alpha -glucosidase and beta -glucosidase and measurement of a fission product. The substrate utilized is a 4-nitrophenyl- beta ,D-maltoheptaoside substituted in the phenyl ring by an electron withdrawing group, preferably a 2-chloro group.